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| 产地 | 中国/美国 |
| 品牌 | KALANG/进口品牌 |
| 货号 | kl-D058Ra01 |
| 保存条件 | -20℃ |
| 英文名称 | Recombinant Neutral Sphingomyelinase (NSMASE) |
| CAS编号 | |
| 保质期 | 12个月个月 |
中性鞘磷脂酶(NSMASE)重组蛋白
Recombinant Neutral Sphingomyelinase (NSMASE)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 66.9kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1μg (determined by the LAL method) |
| Residues | Met1~Ala324 with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
中性鞘磷脂酶(NSMASE)重组蛋白SEQUENCES
The sequence of the target protein is listed below.
MKHNFSLRLR VFNLNCWDIP YLSKHRADRM KRLGDFLNLE SFDLALLEEV WSEQDFQYLK QKLSLTYPDA HYFRSGIIGS GLCVFSRHPI QEIVQHVYTL NGYPYKFYHG DWFCGKAVGL LVLHLSGLVL NAYVTHLHAE YSRQKDIYFA HRVAQAWELA QFIHHTSKKA NVVLLCGDLN MHPKDLGCCL LKEWTGLRDA FVETEDFKGS EDGCTMVPKN CYVSQQDLGP FPFGVRIDYV LYKAVSGFHI CCKTLKTTTG CDPHNGTPFS DHEALMATLC VKHSPPQEDP CSAHGSAERS ALISALREAR TELGRGIAQA RWWA
USAGE
Reconstitute in sterile ddH2O.
中性鞘磷脂酶(NSMASE)重组蛋白STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
中性鞘磷脂酶(NSMASE)重组蛋白About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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