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| 产地 | 中国/美国 |
| 品牌 | KALANG/进口品牌 |
| 货号 | kl-A937Ra01 |
| 保存条件 | -20℃ |
| 英文名称 | Recombinant Nephrin (NPHN) |
| CAS编号 | |
| 保质期 | 12个月个月 |
肾足蛋白(NPHN)重组蛋白
Recombinant Nephrin (NPHN)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 38.4kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1μg (determined by the LAL method) |
| Residues | Pro39~Gly106 linked with KRSECVIVRH with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative |
肾足蛋白(NPHN)重组蛋白SEQUENCES
The sequence of the target protein is listed below.
PV PTSAPRGFWA LSENLTAVEG TTVKLWCGVR APGSVVQWAK DGLLLGPNPK MPGFPRYSLE GDRAKGEFHL LIEACDLSDD AEYECQVGRS ELGPELVSPK VILS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
肾足蛋白(NPHN)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
肾足蛋白(NPHN)重组蛋白About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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