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| 产地 | 中国/美国 |
| 品牌 | KALANG/进口品牌 |
| 货号 | kl-A801Ra01 |
| 保存条件 | -20℃ |
| 英文名称 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) |
| CAS编号 | |
| 保质期 | 12个月个月 |
碱性鞘磷脂磷酸二酯酶(Alk-Smase)重组蛋白
Recombinant Alkaline Sphingomyelinase (Alk-Smase)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 51.5kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1μg (determined by the LAL method) |
| Residues | Gly22~Val439 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
碱性鞘磷脂磷酸二酯酶(Alk-Smase)重组蛋白SEQUENCES
The sequence of the target protein is listed below.
GPVQRQQQH KLLLVSFDGF RWNYDQDVET PNLDSMAQEG VKARYMTPAF VTMTSPCHFT LVTGKYIENH GVVHNMFYNT TNKVRLPYHA TLGIQRWWDN GSIPIWITAQ RQGLKTGSFF YPGGNVTYQG EAVTMSRKEG VLHNYKNETE WRANVDTVMK WFTEEDVSLV TLYFGEPDST GHKYGPESQE RKDMVKQVDR TVGYLRDSIK RHHLTDSLNL IITSDHGMTT VNKKASDLVE FHKFPNFTFR DIEFELLDYG PNGMLIPKEG MLEKVYSVLK DAHPRLHVYK KEDFPKTFHY ANNPRITSLL MYSDLGYVIH GRVNVQFNSG EHGFDNQDMD MKTIFRAVGP SFKAGLEVEP FESVHVYELM CQLLGIVPEP NDGHPGVLQP MLRSGSPLSR QHHLVVVLMG ILTGLAKVV
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
碱性鞘磷脂磷酸二酯酶(Alk-Smase)重组蛋白STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
碱性鞘磷脂磷酸二酯酶(Alk-Smase)重组蛋白About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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