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| 产地 | 中国/美国 |
| 品牌 | KALANG/进口品牌 |
| 货号 | kl-A043Ra01 |
| 保存条件 | -20℃ |
| 英文名称 | Recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF) |
| CAS编号 | |
| 保质期 | 12个月个月 |
胶质细胞系来源神经营养因子(GDNF)重组蛋白
Recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 16.2kDa |
| Concentration | 9.4 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1μg (determined by the LAL method) |
| Residues | Ser78~Ile211 (Accession # Q07731) with N-terminal His-Tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
胶质细胞系来源神经营养因子(GDNF)重组蛋白SEQUENCES
The sequence of the target protein is listed below.
MGHHHHHHSG S- SPD KQAAALPRRE RNRQAAAASP ENSRGKGRRG QRGKNRGCVL TAIHLNVTDL GLGYETKEEL IFRYCSGSCE AAETMYDKIL KNLSRSRRLT SDKVGQACCR PVAFDDDLSF LDDSLVYHIL RKHSAKRCGC I
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
胶质细胞系来源神经营养因子(GDNF)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
胶质细胞系来源神经营养因子(GDNF)重组蛋白About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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